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Skeletal Muscle Differentiation

Genea Biocells developed the world’s first protocol to differentiate human pluripotent stem cells to skeletal muscle with high yields and without cell sorting or genetic manipulation. Myotubes are contractile, express typical muscle markers and show striated sarcomeres. The process yields almost pure populations of myogenic cells; it is highly efficient and works robustly with all stem cell lines – critical prerequisites to use this technology effectively for disease modeling and drug development.

Muscle is generated from human pluripotent stem cells in 3 steps, via satellite-like or progenitor cells and myoblasts that then fuse to multinucleated myotubes in the third step. Our third generation of the process includes an optional fusion enhance that replaces the regular third step.